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Posted on: 09/12/2016

EXTRACTING DNA

To demonstrate that fruit are living things, Year 8 Biology pupils were set the challenge of extracting DNA from kiwi fruit. This is what they did:

  1. Carefully peel the skin off a quarter (15g) of a kiwi fruit (chop it into tiny pieces)
  2. Put the pieces into a pestal and mortar and mash up carefully
  3. Place the mashed kiwi in a test tube
  4. Add a spatula of salt and approximately 4-5cm of washing up liquid/pineapple juice to the boiling tube
  5. Leave the tube to stand in a beaker of water at 60°C for 5-7 minutes. While you are waiting, place filter paper in a funnel in a test tube
  6. Then move the kiwi sample  from the beaker of hot water to an ice bath and leave for five minutes
  7. Drain the contents of your tube through the filter paper so that the green liquid goes into the clean boiling tube
  8. Take the cooled tube to the teacher who will pour ice cold ethanol down the side of the tube
  9. Your kiwi DNA will float out of the mixture and you can observe it using the glass rod.

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